Removal of Paracetamol by Peroxidase from Cauliflower Stem
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Published:
Apr 28, 2017
Keywords:
Paracetamol Peroxidase Cauliflower stem Removal
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Abstract
This research aims to study the removal of paracetamol by peroxidase extracted from cauliflower stems. The concentration of enzyme, the concentration of paracetamol, the concentration of hydrogen peroxide, pH of the solution and temperature were tested. The experiments were carried out in batch system at stirrer speed 200 rpm. The results showed that the maximum removal efficiency of paracetamol was 66.47% at paracetamol concentration 1 mg/l, enzyme concentration 0.124 U/ml, hydrogen peroxide 1 mM, pH of the solution at 7 and 25 0C. The study indicated the feasibility of peroxidase for removal of paracetamol contaminated in water and can be applied in the industries and hospitals.
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Research Articles
References
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[2] Hamid, M. and Khalil ur, R. 2009. Potential applications of peroxidases. Food Chem. 115: 1177-1186.
[3] Alemzadeh, I. and Nejati, S. 2009. Phenols removal by immobilized horseradish peroxidase. J. Hazard. Mater. 166: 1082-1086.
[4] Nagenda, P. 2011. Spectrophotometric Estimation of Paracetamol in Bulk and Pharmaceutical Formulations. E-Journal of Chemistry. 8: 149-152.
[5] Singh, S., Melo, J.S., Eapen, S. and D'Souza, S.F. 2006. Phenol removal using Brassica juncea hairy roots: Role of inherent peroxidase and H2O2. J. Biotechnol. 123: 43-49.
[6] Deva, A.N., Arun, C., Arthanareeswaran, G. and Sivashanmugam, P. 2014. Extraction of peroxidase from waste Brassica oleracea used for the treatment of aqueous phenol in synthetic waste water. J. Envi Chem. Eng. 2: 1148-1154.
[7] Bódalo, A., Gómez, J.L., Gómez, E., Hidalgo, A.M., Gómez, M. and Yelo, A.M. 2006. Removal of 4-chlorophenol by soybean peroxidase and hydrogen peroxide in a discontinuous tank reactor. Desalination. 195: 51-59.
[8] Bayramoğlu, G. and Arıca, M.Y. 2008. Enzymatic removal of phenol and p-chlorophenol in enzyme reactor: Horseradish peroxidase immobilized on magnetic beads. J. Hazard. Mater. 156: 148-155.
[9] Ba, S., Haroune, L., Cruz-Morató, C., Jacquet, C., Touahar, I.E., Bellenger, J.-P., Legault, C.Y., Jones, J.P. and Cabana, H. 2014. Synthesis and characterization of combined cross-linked laccase and tyrosinase aggregates transforming acetaminophen as a model phenolic compound in wastewaters. Sci. Total Environ. 487: 748-755.