Effect of Salinity on Chlorella vulgaris for Increasing Lipid Content
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Abstract
Microalgae growth with effluent from the frozen seafood wastewater treatment plant can provide some benefits such as produce high biomass and lipid for biodiesel production. Chlorella vulgaris was considered as the best feedstock of energy to produce biodiesel. The objective of this study was to increase lipid content under salinity stress. There were three reactors including R1, R2 and R3 for cultivating microalgae. The R1 used the effluent of seafood processing wastewater treatment plant without adding the NaCl. The R2 and R3 were added with the NaCl for increasing the salinity. The various NaCl concentrations of R1, R2 and R3 were 1.34 g/L (0.023 M of NaCl), 2.9 g/L (0.050 M of NaCl) and 4.4 g/L (0.075 M of NaCl), respectively. In this study, the Chlorella vulgaris growth in R1 and R2 was reached the maximum of dry cell weight (DCW) about 1.02 g/L and 1.16 g/L on Day-3, respectively. While the Chlorella vulgaris growth in R3 was reached the maximum of DCW about 1.47 g/L on Day-4. The total lipid content of Chlorella vulgaris was increased in different concentration of salinity. The total lipid content in R2 was lower than in R3, of which R2 and R3 contained 1.84% and 3.09% of lipid content, respectively. However, both reactors were lower than the lipid content of R1 which was 4.60% of lipid content. It could be concluded that the lipid content in Chlorella vulgaris strain was enhanced slightly between various concentrations of salinity. Therefore, the effluent from frozen seafood industry was suitable for growth Chlorella vulgaris without adding NaCl. The salinity content in the effluent from frozen seafood industry (0.023 M of NaCl) was enough for microalgae growth and the nutrient contained in the effluent, was also removed from microalgae cultivation.
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