Ammonium-Nitrogen Removal in Wastewater Through Adsorption Utilizing Bio-Sorbent Matrix

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Chalisa Jantaraksa
Nawatch Surinkul


Adsorption is one of the effective methods for removing nutrients from effluent. Adsorbent such as chitosan and various formed of activated carbon such as granulated, maize cob and bamboo were used in nitrogen-rich wastewater treatment. An alternative to the aforementioned adsorbent is bio-sorbent, a biological material that could adsorb desired substances from aqueous solution. Eggshell, with calcium carbonate as the main composition, is a waste and readily available biological material for the adsorbent matrix development. This study aimed to develop an effective and economical alternative adsorbents matrix with eggshell incorporated for the adsorption of nitrogen for the treatment of effluent from aquaculture. Eggshell was collected and washed with distilled water several times and dried in the oven at 105oC overnight, then, ground into different sizes of 250-151, 150-75, and less than 75 µm. Batch experiments were performed under a simulated influent condition with ammonium sulfate concentration of 1 to 10 gL-1. The ammonium removals were ranged from 20 to 44 percent. Formed eggshell matrixes with bentonite was also tested for ammonium nitrogen adsorption, the adsorption efficiency were ranged from 20 to 41 percent. This study reaffirms that eggshell matrix could be used as bio-sorbent for ammonium nitrogen removals. However, the treatment cost of eggshell is needed to be considered.

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Ebeling, J. M., Timmons, M. B. and Bisogni, J. J. 2006. Engineering analysis of the stoichiometry of photoautotrophic, autotrophic, and heterotrophic removal of ammonia–nitrogen in aquaculture systems. Aquaculture. 257(1-4): 346-358.

Ferrari, S., Junior, E. F., Godoy, L. J. G. D., Ferrari, J. V., Souza, W. J. O. D., and Alves, E. 2014. Effects on soil chemical attributes and cotton yield from ammonium sulfate and cover crops. Acta Scientiarum. Agronomy. 37(1): 75-83. doi: 10.4025/actasciagron.v37i1.17972

Watson, C. J. 1988. An assessment of granular urea/ammonium sulphate and urea/potassium nitrate fertilizers on nitrogen recovery by ryegrass. Fertilizer Research. 18(1): 19-29. doi: 10.1007/bf01064175.

Casteel, S. N., Chien, S. H. and Gearhart, M. M. 2019. Field Evaluation of Ammonium Sulfate versus Two Fertilizer Products Containing Ammonium Sulfate and Elemental Sulfur on Soybeans. Communications in Soil Science and Plant Analysis. 50(22): 2941-2947. doi: 10.1080/00103624.2019.1689257

Ishikawa, N., Ishioka, G., Yanaka, M., Takata, K. and Murakami, M. 2015. Effects of Ammonium Chloride Fertilizer and its Application Stage on Cadmium Concentrations in Wheat (Triticum aestivumL.) Grain. Plant Production Science. 18(2): 137-145. doi: 10.1626/pps.18.137

Liu, H., Dong1, Y., Wang, H. and Liu, Y. 2010. Adsorption behavior of ammonium by a bioadsorbent – Boston ivy leaf powder. Journal of Environmental Sciences. 22(10): 1513-1518. doi: 10.1016/S1001-0742(09)60282-5

La, J., Kim, T., Jang, J. K. and Chang, I. S. 2014. Ammonia Nitrogen Removal and Recovery from Swine Wastewater by Microwave Radiation. Environmental Engineering Research. 19(4): 381-385. doi: 10.4491/eer.2014.064

Withers, P., Neal, C., Jarvie, H. and Doody, D. 2014. Agriculture and Eutrophication: Where Do We Go from Here? Sustainability. 6(9): 5853-5875. doi:10.3390/su6095853

Zadinelo, I. V., Santos, L. D., Cagol, L., Muniz, G. I., Ellendersen, L. D., Alves, H. J. and Bombardelli, R. A. 2017. Adsorption of aquaculture pollutants using a sustainable biopolymer. Environmental Science and Pollution Research. 25(5): 4361-4370. doi:10.1007/s11356-017-0794-4

Chen, D., Xiao, X. and Yang, K. 2016. Removal of phosphate and hexavalent chromium from aqueous solutions by engineered waste eggshell. RSC Advances. 6(42): 35332-35339. doi:10.1039/c6ra05034d

Taylor, D. P., Smith, S. A. and Kuhn, D. D. 2017. Adsorptive performance of granular activated carbon in aquaculture and aquaria: A simplified method. Journal of Applied Aquaculture. 29(3-4): 291-306. doi:10.1080/10454438.2017.1384780

Lo, S., Wang, S., Tsai, M. and Lin, L. 2012. Adsorption capacity and removal efficiency of heavy metal ions by Moso and Ma bamboo activated carbons. Chemical Engineering Research and Design. 90(9): 1397-1406. doi:10.1016/j.cherd.2011.11.020

Bernardi, F., Zadinelo, I. V., Alves, H. J., Meurer, F. and Santos, L. D. 2018. Chitins and chitosans for the removal of total ammonia of aquaculture effluents. Aquaculture. 483: 203-212. doi:10.1016/j.aquaculture.2017.10.027

Michalak, I., Chojnacka, K. and Witek-Krowiak, A. 2013. State of the Art for the Biosorption Process—a Review. Applied Biochemistry and Biotechnology. 170(6): 1389-1416. doi:10.1007/s12010-013-0269-0

Olivares-Marín, M., Cuerda-Correa, E.M., Nieto-Sánchez, A., Garcia, S., Pevida, C. and Román, S. 2012. Influence of morphology, porosity and crystal structure of CaCO3 precursors on the CO2 capture performance of CaO-derived sorbents. Chemical Engineering Journal. 217: 71-81. doi:

Gao, Y. and Xu, C. 2012. Synthesis of dimethyl carbonate over waste eggshell catalyst. Catalysis Today. 190: 107-111. doi:

Ehrampoush, M., Ghanizadeh, G. and Ghaneian, M. 2011. Equilibrium and kinetics study of reactive red 123 dye removal from aqueous solution by adsorption on eggshell. Iranian Journal of Environmental Health Science & Engineering. 8: 101-108.

Tsai, W., Yang, J., Lai, C., Cheng, Y., Lin, C. and Yeh, C. 2006. Characterization and adsorption properties of eggshells and eggshell membrane. Bioresource Technology, 97(3), 488-493. doi:10.1016/j.biortech.2005.02.050

Mezenner, N. Y. and Bensmaili, A. 2009. Kinetics and thermodynamic study of phosphate adsorption on iron hydroxide-eggshell waste. Chemical Engineering Journal. 147(2-3): 87-96. doi:10.1016/j.cej.


Mignardi, S., Archilletti, L., Medeghini, L. and Vito, C. D. 2020. Valorization of Eggshell Biowaste for Sustainable Environmental Remediation. Scientific Reports. 10(1): 1-10. doi: 10.1038/s41598-020-59324-5

Zamparas, M., Drosos, M., Georgiou, Y., Deligiannakis, Y. and Zacharias, I. 2013. A novel bentonite-humic acid composite material Bephos™ for removal of phosphate and ammonium from eutrophic waters. Chemical Engineering Journal. 225: 43-51. doi: 10.1016/j.cej.2013.03.064

Stathi, P., Papadas, I. T., Enotiadis, A., Gengler, R. Y. N., Gournis, D., Rudolf, P. and Deligiannakis, Y. 2009. Effects of Acetate on Cation Exchange Capacity of a Zn-Containing Montmorillonite: Physicochemical Significance and Metal Uptake. Langmuir. 25(12): 6825-6833. doi: 10.1021/la900831q

Elkhalifah, A. E., Maitra, S., Bustam, M. A. and Murugesan, T. 2013. Effects of exchanged ammonium cations on structure characteristics and CO2 adsorption capacities of bentonite clay. Applied Clay Science. 83-84: 391-398. doi: 10.1016/j.clay.2013.07.016

American Public Health Association. 2017. Standard methods for the examination of water and wastewater (23rd ed.). American Public Health Association, Washington, DC.

Chuiprasert, J. and Surinkul, N. Development of Activated Adsorbent from Water Treatment Sludge: Application for Constructed Wetland Media Treating Ammonium Nitrogen. At The 12th International Symposium on Southeast Asian Water Environment (SEAWE2AL6) Hanoi, Vietnam, November 28-30, 2016.

Zhao, Y., Yang, Y., Yang, S., Wang, Q., Feng, C. and Zhang, Z. 2013. Adsorption of high ammonium nitrogen from wastewater using a novel ceramic adsorbent and the evaluation of the ammonium-adsorbed-ceramic as fertilizer. Journal of Colloid and Interface Science. 393: 264-270. doi: 10.1016/j.jcis.2012.10.028