Selection of the Non-methanogenic Microorganism for Biogas Production from Napier Grass Extract

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Selyne KOK
Peerakarn Banjerdkij
Supaporn Panwilai
Siriton Teeravet


This study selected outward methanotrophic bacteria for biogas potential experiments in Napier at the age of 60 and over 90 days using the Biochemical Methane Potential method (BMP) until steady state at mesophilic temperature. In this experiment, Clostridium beijerinckii and Cutibacterium acnes were chosen because they both have biogas potential in extracted Napier at the age of 60 and over 90 days of 59.98% maximal methane production. The accumulation of methane production was 4.84, 1.02, and 0.53 ml, respectively, as a result of Napier extract of 60 days with Clostridium beijerinckii (N60G), 90 days with Clostridium beijerinckii (N90G), and 90 days with Cutibacterium acnes (N90K), and 0.28, 2.26, and 0.33 ml/TSadded of BMP.


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