Performance of Synthetic Urine-Fed Microbial Fuel Cell at Various Substrate Concentrations and Flow Rates

Main Article Content

Warista Chumroen
Petch Pengchai

Abstract

The purpose of this study was to evaluate the performance of microbial fuel cells (MFCs) fed with urine at various substrate concentrations and flow rates. In this work, 3 MFCs were used to process synthetic human urine (SU-D0) and diluted synthetic human urine (SU-D1) at flow rates of 20 L/d (MFC1, HRT=6h), 30 L/d (MFC2, HRT=4h), and 40 L/d (MFC3, HRT=3h). The results showed that MFC2 was the best energy producer (1.221±1.579 mW/m2 for SU-D0, 0.153 ±0.133 mW/m2 for SU-D1) and the best nutrient remover due to its maximum removal efficiencies in both SU–D0 (45.6±2.8% for NO3-, 41.2±20.0% for NO2-, 40.6±12.8% for TN, 36.1±27.7% for PO43-) and SU-D1 (39.0±32.4% for NO3-, 31.4±10.3% for PO43-) conditions. The modified Lineweaver-Burk plot with the determination coefficient (R2) of 0.922-0.975 revealed that the increased substrate loading rate contributed to the higher nutrient removal rate. Furthermore, this study found that the power densities and the removal efficiencies of NO3-, NO2-, and PO43- were positively correlated.

Article Details

Section
Research Articles
Author Biography

Warista Chumroen, Environmental Engineering Laboratory, Circular Resources and Environmental Protection Technology Research Unit (CREPT), Faculty of engineering, Mahasarakham University

Environmental Engineering Laboratory, Circular Resources and Environmental Protection Technology Research Unit (CREPT), Faculty of engineering, Mahasarakham University, Mahasarakham 44150, Thailand

References

Santoro, C., Garcia, M.J.S., Walter, X.A., You, J., Theodosiou, P., Gajda, I., Obata, O., Winfield, J., Greenman,

J. and Ieropoulos, I. 1995. Urine in Bioelectrochemical Systems: An Overall Review. Chem Electro Chem. 10.1002/celc.201901995. Accessed: May. 18, 2022 [Online]. Available: https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/celc.201901995.

Landry, K.A. and Boyer, T.H. 2016. Life Cycle Assessment and Costing of Urine Source Separation: Focus on Nonsteroidal Antiinflammatory Drug Removal. Water Research. 105: 487-495.

You, J., Greenman, J., Melhuish, C. and Ieropoulos, I. 2014. Electricity Generation and Struvite Recovery from Human

Urine Using Microbial Fuel Cells. Journal of Chemical Technology and Biotechnology. 91(3): 647-654.

Ieropoulos, I., Greenman, J. and Melhuish, C. 2012. Urine Utilisation by Microbial Fuel Cells; Energy Fuel for The Future. Physical Chemistry Chemical Physics. 14: 94-98.

Walter, X.A., Stinchcombe, A., Greenman, J. and Ieropoulos, I. 2017. Urine Transduction to Usable Energy: A Modular MFC Approach for Smartphone and Remote System Charging. Applied Energy. 192: 575-581.

Walter, X.A., Merino-Jiménez, I., Greenman, J. and Ieropoulos, I. 2018. PEE POWER® urinal II – Urinal Scale-up with Microbial Fuel Cell Scale-down for Improved Lighting. Journal of Power Sources. 392: 150-158.

Walter, X.A., Greenman, J. and Ieropoulos, I. 2020. Microbial Fuel Cells Directly Powering a Microcomputer. Journal of Power Sources. 446: 227328.

Silvester, K.R., Bingham, S.A., Pollock, J.R., Cummings, J.H. and O’Neill, I.K. 1997. Effect of Meat and Resistant Starch on Fecal Excretion of Apparent N-nitroso Compounds and Ammonia from the Human Large Bowel. Nutr. Cancer. 29: 13-23.

Udert, K.M., Larsen, T.A., Biebow, M. and Gujer, W. 2003. Urea Hydrolysis and Precipitation Dynamics in a Urine-Collecting System. Water Research. 37: 2571-2582.

Udert, K.M., Larsen, T.A. and Gujer, W. 2003. Biologically Induced Precipitation in Urine-collecting Systems. Water Sci. Technol. Water Supply. 3(3): 71-78.

Larsen, T.A. and Gujer, W. 1996. Separate Management of Anthropogenic Nutrient Solutions (Human Urine). Water Science and Technology. 34(3-4): 87-94.

Udert, K.M., Larsen, T.A. and Gujer, W. 2003. Estimating the Precipitation Potential in Urine-collecting Systems. Water Research. 37: 2667-2677.

You, J., Greenman, J., Melhuish, C. and Ieropoulos, I. 2016. Electricity Generation and Struvite Recovery from Human Urine Using Microbial Fuel Cells. Journal of Chemical Technology and Biotechnology. 91: 647-654.

Potrykus S., Mateo S., Nieznnski J. and Fernández-Morales F.J. 2020. The Influent Effects of Flow Rate Profile on the Performance of Microbial Fuel Cells Model. Energies. 13(18): 4735.

Ni H., Wang K., Lv S., Wang X., Zhuo L. and Zhang J. 2020. Effects of Concentration Variations on the Performance and Microbial Community in Microbial Fuel Cell Using Swine Wastewater. Energies. 13(9): 2231.

Sukkasem, C., Laehlah, S., Hniman, A., O’thong, S., Boonsawang, P., Rarngnarong, A., Nisoa, M. and Kirdtongmee, P. 2011. Upflow Bio-filter Circuit (UBFC): Biocatalyst Microbial Fuel Cell (MFC) Configuration and Application to Biodiesel Wastewater Treatment. Bioresource Technology. 102(22): 10363-10370.

Choudhury, P., Uday, U.S., Bandyopadhyay, T.K., Ray R.N. and Bhunia, B. 2017. Performance Improvement of Microbial Fuel Cell (MFC) Using Suitable Electrode and Bioengineered Organisms: A Review. Bioengineered. 8(5): 471-487.

McLean, R.J.C., Nickel, J.C., Cheng, K.-J., Costerton, J.W. and Banwell, J.G. 1988. The Ecology and Pathogenicity of Urease-producing Bacteria in the Urinary Tract. CRC Critical Reviews in Microbiology. 16(1): 37-79. Accessed: Jan. 2, 2023 [Online]. Available: https://scholar.google.com/citations?view_op=view_citation&hl=en&user=qDVk4ZAAAAAJ&cstart=100&pagesize=100&sortby=pubdate&citation_for_view=qDVk4ZAAAAAJ:zYLM7Y9cAGgC.

American Public Health Association (APHA). 2017. Standard Methods for the Examination of Water and Wastewater. American Water Works Association, 22rd ed. Washington D.C., New York.

Kaul, S.N. 2002. Water and Wastewater Analysis. Daya Publishing House, New Delhi.

U.S. Environmental Protection Agency (USEPA). 1993. Method 350.1 Determination of ammonia nitrogen by semi-automated colorimetry. 2nd revision. Environmental Monitoring Systems Laboratory, Office of Research and Development, Ohio.

Gou Y. 2001. Determination of total nitrogen in water samples by spectrophotometry using phenol after alkaline peroxodisulfate digestion. Bunseki Kagaku. 50(7): 481-486.

Dowd, J.E. and Riggs, D.X. 1965. Comparison of Estimates of Michaelis-Menten Kinetic Constants from Various Linear Transformations. The Journal of Biological Chemistry. 240(2): 863-869.

Puyol, D., Carvajal-Arroyo, J M, Li, G.B., Dougless, A., Fuentes-Velasco, M., Sierra-Alvarez, R. and Field, J.A. 2014. Field. High pH (and not Free Ammonia) Is Responsible for Anammox Inhibition in Mildly Alkaline Solutions with Excess of Ammonium. Biotechnology Letters. 36(10): 1981-1986.

Kim, S.H., Song, S.H. and Yoo, Y.J. 2004. The pH as a Control Parameter for Oxidation-Reduction Potential on the Denitrification by Ochrobactrum anthropi SY 509. Journal of Microbiology and Biotechnology. 14(3): 639-642.

Mongkulphit, S., Pengchai, P. and Suwannata N. 2021. Influence of Very High Flow Rates on Performance of Biofilter-Microbial Fuel Cells. International Journal of Environmental Science and Development. 12(3): 69-74.

Robinson, P.K. 2015. Enzymes: principles and biotechnological applications. Essays Biochem. 59: 1-41. Accessed: Jan. 2,

[Online]. Available: https://portlandpress.com/essaysbiochem/article/doi/10.1042/bse0590001/88345/Enzymes-principles-and-biotechnological.

Aslanzadeh, S., Ishola, M., Richards, T. and Taherzadeh, M. 2014. An Overview of Existing Individual Unit Operations. Biorefineries. Integrated Biochemical Processes for Liquid Biofuels. 3-36. Accessed: Jan. 2, 2023 [Online]. Available: https://www.researchgate.net/publication/289707814_An_Overview_of_Existing_Individual_Unit_Operations.

Guo Y., Wang J., Shinde S., Wang X., Li Y., Dai Y., Ren J., Zhang, P. and Liu, X. 2020. Simultaneous Wastewater Treatment and Energy Harvesting in Microbial Fuel Cells: An Update on the Biocatalysts. RSC Advances. 10: 25874-25887.

Paucar, N.E. and Sato, C. 2021. Microbial Fuel Cell for Energy Production, Nutrient Removal and Recovery from Wastewater: A Review. Processes. 9(8): 1318.

Liao, M., Yuan, L., Enling, T., Weiqi, M. and Hong, L. 2020. Phosphorous Removal and High-purity Struvite Recovery from Hydrolyzed Urine with Spontaneous Electricity Production in Mg-air Fuel Cell. Chemical Engineering Journal. 391(1): 123517.