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The biogas production from organic wastes from the recirculating aquaculture systems was conducted in this study. The experiment aimed to evaluate biogas production potential with 3 types of organic wastes including (1) Biofloc ; suspended solid collected from biofloc aquaculture system (2) Feces ; fish feces was separate from recirculating aquaculture tank by continuous sludge separator and (3) Sludge ; aquaculture sludge that consist feces and uneaten feed from recirculating aquaculture tank. Moreover, the optimal ratio between organic waste and biogas inoculum i.e. 1:4, 2:3, 1:1, 3:2 and 4:1 were evaluated as to provide the maximum biogas yield. The experiment was conducted in 100 ml serum bottles using Biochemical Methane Potential (BMP) technique for 42 days. It was found that feces had the highest biogas and methane production of 347.65 + 32.95 mL/g VS substrate and 175.28 + 16.62 mL CH4/g VS substrate, respectively. The optimal substrate to inoculum ratio at 3:2 gave the highest biogas production rate of 361.93 + 3.68 mL/g VS substrate with 58.23% methane content.
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