Isolation of Bacteria and Epizoite Barnacle from Portunid Crab (Portunidae: Decapoda) at Kung Krabaen Bay, Chanthaburi Province
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Isolation of bacteria and epizoite barnacles from portunid crab (Portunidae: Decapoda); blue swimming crab Portunus pelagicus, mud crab Scylla transquebarica and crucifix crab Charybdis feriatus was conducted from January to December 2015 at Kung Krabaen Bay by using collapsible crab trap and crab gill nets. The bacteria was isolated from five organs; muscle, gill, heart, hepatopancrease and stomach. Morphology of bacteria was observed under compound microscopes and biochemical tests were also studied. Moreover, the research identified the epizoite barnacles and localized crabs position including influence of factors that had effected on epizoite barnacles such as sex, size and season were carried out. The result found that 6 generas (11 isolates), 3 generas (10 isolates), and 5 generas (9 isolates), of bacteria were isolated from Portunus pelagicus, Scylla transquebarica and Charybdis feriatus, respectively. The Vibrio sp. was the most dominant generas. The bacteria was mostly found in the gill, hepatopancrease and stomach in P. pelagicus, S. transquebarica and C. feriatus. Octolasmis sp. was found in all species especially in the gill. Chelonibia sp. was found in P. pelagicus and S. transquebarica, in particularly, it was walking legs and in carapaces. In addition, this research was found that sex, size and season were no significance to abundance of epizoite barnacles in the host crabs. However, season of C. feriatus indicate significant difference to the habitat of epizoite barnacle that lived in crab (p<0.05). Epizoite barnacles usually lived in the crab in dry seasons better than rainy seasons.
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