Effects of Sucrose on In vitro Slow Growth of Dendrobium hercoglossum Rchb. f.

Abstract

 The study on the effects of sucrose on in vitro slow growth of Dendrobium hercoglossum Rchb. f. seedling for prolong subculture with modified Vacin and Went (1949) media supplementation, different concentrations of sucrose (0, 2, 4, 6, 8 and 10 percent) were used. The experiment was conducted following the Completely Randomized Design (CRD) with 6 treatments in each treatment consisted of ten replicates with four explants per bottle. Results showed that plant height was reduced when applied with 10 percent of sucrose (1.210 cm) and could preserve for 6 months without subculture when compared with basic medium (4 percent of sucrose). The highest plant height (1.725 cm) and widest pseudo-bulb diameter (0.215 cm) were obtained from seedling cultured on Vacin and Went (1949) medium containing 4 percent of sucrose.